FastQCFastQC Report
Fri 10 Feb 2017
ERR1148149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719579
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49000.28495346826170825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26760.15561948593231248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.15358410401615744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.056.8041841
GTATCAA42600.050.602111
AACGCAG50900.042.4256976
TCAACGC51400.041.43434
CAACGCA52750.040.599455
ATCAACG53350.039.8083273
TATCAAC59200.035.978192
ACGCAGA60050.035.763037
CGCAGAG63000.034.182848
GTACATG68500.032.251731
ACATGGG68550.031.2417373
GCAGAGT69850.031.0861249
TACATGG70700.031.0516722
GTATACG1055.8788556E-428.3563561
CATGGGG39350.026.6077424
AGAGTAC66300.025.88644610-11
CGTATAG1951.4551824E-624.430091
CAGAGTA69650.024.12889710-11
GAGTACT32800.023.3060912-13
CATGGGT15000.023.0025964
Produced by FastQC (version 0.11.5)