FastQCFastQC Report
Fri 10 Feb 2017
ERR1148148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397394
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101600.4237935024447379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54150.22587025745455275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50510.21068710441420976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76850.055.5569041
GGTATCA41350.044.2105181
AACGCAG96000.043.4977156
TCAACGC103800.040.572944
CAACGCA104950.040.1850365
ATCAACG105250.040.0704963
TATCAAC109350.039.0584752
ACGCAGA111050.037.6562737
CGCAGAG114050.036.5614438
GTACATG118650.036.2353321
TACATGG122750.035.0369572
ACATGGG120200.034.987713
GCAGAGT125800.033.241099
CATGGGG68150.032.382374
ATGGGGG30650.032.022415
AGAGTAC119150.029.70458410-11
CATGGGT26000.028.5981034
GAGTACT60050.025.16061612-13
TGGGGGG25650.025.0459186
AGTACTT66900.024.80725312-13
Produced by FastQC (version 0.11.5)