FastQCFastQC Report
Fri 10 Feb 2017
ERR1148147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025527
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79140.390713132927875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.1967389227593609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38060.1879017164421901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61150.057.7555731
GGTATCA31600.052.395421
TCAACGC80500.043.7457474
AACGCAG79900.043.4786536
CAACGCA81750.042.858565
ATCAACG82600.042.6335643
TATCAAC87500.040.4520382
ACGCAGA91400.037.943067
CGCAGAG92650.037.3027428
GTACATG95850.034.9204521
GCAGAGT101250.034.1930589
TACATGG98050.033.975972
ACATGGG96550.033.454733
AGAGTAC94950.029.31979210-11
CATGGGG55500.028.9388184
CATGGGT20600.027.7213294
AGTACTT50050.026.14740612-13
CAGAGTA99400.025.8827110-11
GAGTACT46350.025.60372512-13
ATGGGGG26900.024.988275
Produced by FastQC (version 0.11.5)