FastQCFastQC Report
Fri 10 Feb 2017
ERR1148146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487946
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86730.3486008136832552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48110.1933723641911842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.17339604637721237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72650.057.865181
GGTATCA36100.048.9888731
AACGCAG93300.044.6265226
TCAACGC96500.043.269954
CAACGCA98450.042.4129075
ATCAACG98800.042.142253
TATCAAC107600.039.1947672
ACGCAGA106350.039.1504867
CGCAGAG108100.038.461678
GTACATG114250.035.961771
GCAGAGT118300.035.1454479
TACATGG118350.034.679672
ACATGGG116800.034.5782933
CATGGGT30100.031.81534
AGAGTAC111250.030.4220810-11
CATGGGG65600.029.9217324
ATGGGGG29450.026.6603155
AGTACTT59250.025.74990512-13
CAGAGTA116300.025.4442710-11
GAGTACT53000.024.9145912-13
Produced by FastQC (version 0.11.5)