FastQCFastQC Report
Fri 10 Feb 2017
ERR1148145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928935
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94530.3227452982056618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49280.16825228282635157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43860.14974726308368058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76550.058.4167141
GGTATCA37650.045.7062151
TCAACGC98900.044.2683874
AACGCAG98950.044.1257826
CAACGCA100850.043.412435
ATCAACG102300.042.91343
TATCAAC113150.039.171772
ACGCAGA114650.038.0832637
CGCAGAG119450.036.552928
GTACATG127700.035.577551
TACATGG131000.034.4700552
ACATGGG127950.034.3570863
GCAGAGT130450.033.425059
CATGGGG70500.030.7131544
AGAGTAC119600.029.12110710-11
ATGGGGG34750.025.6772565
AGTACTT61900.024.74564412-13
GAGTACT56600.024.43535612-13
CAGAGTA125550.024.3533410-11
CATGGGT34800.023.760074
Produced by FastQC (version 0.11.5)