FastQCFastQC Report
Fri 10 Feb 2017
ERR1148144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533233
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48650.317303371372779No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.16651089560425586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.15666242508477185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36150.061.3098831
GGTATCA18900.048.5461461
TCAACGC50950.043.0864034
CAACGCA51850.042.338525
AACGCAG52100.042.1353576
ATCAACG52400.042.007663
GTACATG75500.038.66741
TATCAAC57750.038.0192222
TACATGG77900.036.892542
ACATGGG76850.036.538963
ACGCAGA61800.035.6181457
CGCAGAG64750.033.9953848
CATGGGT20450.032.5823754
GCAGAGT70300.031.5654189
CATGGGG45850.031.1408144
AGAGTAC65400.029.15468410-11
ATGGGGG26200.027.0211435
GTAAGTA9150.024.7110612
AGTACTT31400.024.63041912-13
GTATAGG3252.3646862E-1123.8317431
Produced by FastQC (version 0.11.5)