FastQCFastQC Report
Fri 10 Feb 2017
ERR1148143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678954
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61140.3641553014555491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.20149450193394222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.17951653231714507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44900.059.0106851
GGTATCA22750.052.605561
TCAACGC58000.044.822414
CAACGCA59050.043.9246565
ATCAACG59850.043.6357153
AACGCAG60300.042.915456
TATCAAC64450.040.798212
ACGCAGA71750.036.0669257
CGCAGAG73900.035.0981148
GTACATG79950.032.8425671
TACATGG81950.031.8681852
ACATGGG80600.031.5900613
GCAGAGT83200.031.5323989
TAGACCG1802.2033419E-829.744855
CATGGGG47900.029.3101654
ATGGGGG24500.027.6809185
AGAGTAC76650.026.89260110-11
GTATTAG8750.026.5383261
AGTACTT36750.024.11963312-13
CAGAGTA80100.023.9889810-11
Produced by FastQC (version 0.11.5)