FastQCFastQC Report
Fri 10 Feb 2017
ERR1148142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969191
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76480.38838284351289437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39570.20094546440644914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.19871104428163647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57250.062.122531
GGTATCA27650.048.0463141
AACGCAG73200.047.6220256
TCAACGC75500.046.5652434
CAACGCA77300.045.5578845
ATCAACG77950.045.1016733
TATCAAC83250.042.521562
ACGCAGA84000.041.4991957
CGCAGAG87450.039.9300278
GCAGAGT96800.036.3189629
GTACATG92050.034.81841
ACATGGG92350.033.9465033
TACATGG95650.033.2148862
CATGGGG53400.030.1890434
AGAGTAC90850.029.82529610-11
CATGGGT20600.029.743454
AGTACTT47200.025.8364712-13
CAGAGTA93550.025.65789810-11
GAGTACT43850.025.36841612-13
ATGGGGG23900.024.3921175
Produced by FastQC (version 0.11.5)