FastQCFastQC Report
Fri 10 Feb 2017
ERR1148141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465834
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113080.32627067539876403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59090.17049287415381117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57380.1655589967667234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83800.056.4956361
GGTATCA42850.050.4484251
AACGCAG107300.043.295386
TCAACGC109400.042.5730444
ATCAACG111200.041.883913
CAACGCA111900.041.5687525
TATCAAC123450.038.0683332
ACGCAGA123150.037.867967
CGCAGAG127250.036.601118
GTACATG125650.034.7402761
GCAGAGT140750.033.3018269
TACATGG133400.032.6870732
ACATGGG130300.032.4575273
CATGGGG74150.029.9218164
AGAGTAC130500.029.33109710-11
CAGAGTA136800.024.9366210-11
AGTACTT78050.024.80666412-13
GAGTACT68600.024.23527712-13
CATGGGT31350.023.1479514
TATACAG19150.021.7430045
Produced by FastQC (version 0.11.5)