FastQCFastQC Report
Fri 10 Feb 2017
ERR1148140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3645039
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127580.35000997245845655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66510.18246718347869528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62750.1721517931632556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98900.059.3160441
GGTATCA48600.047.3024061
AACGCAG124650.046.3854376
TCAACGC128250.045.1761554
CAACGCA130950.044.3809625
ATCAACG132000.043.982873
TATCAAC140300.041.5951652
ACGCAGA141900.040.7046977
CGCAGAG144450.039.944958
GCAGAGT164100.035.3067749
GTACATG149800.034.8674351
TACATGG158900.032.8701822
ACATGGG155650.032.7521173
AGAGTAC151600.030.17413510-11
CATGGGG87250.030.1345354
CAGAGTA159700.025.23550810-11
AGTACTT86650.024.61094512-13
CATGGGT38150.024.4800764
GAGTACT76100.023.87999512-13
ATGGGGG40600.023.7354035
Produced by FastQC (version 0.11.5)