FastQCFastQC Report
Fri 10 Feb 2017
ERR1148139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4146390
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88180.21266692231073295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42510.10252291752584777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.10155822293609622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87000.057.633191
TCAACGC103550.047.6208274
AACGCAG104100.047.1978076
GGTATCA41200.047.1194461
CAACGCA106850.046.0944145
ATCAACG108100.045.561413
ACGCAGA117500.041.865877
TATCAAC123300.040.3818552
CGCAGAG122850.039.9942368
GCAGAGT141050.034.875869
GTACATG137000.033.0783541
ACATGGG134450.032.207953
TACATGG144800.031.0582622
AGAGTAC131400.029.01717610-11
CATGGGG74650.028.4466344
CATGGGT35650.025.1947424
CAGAGTA140100.024.60411810-11
GAGTACT64850.023.80235312-13
ATGGGGG37000.022.5070635
GTATAGG13700.021.7334791
Produced by FastQC (version 0.11.5)