FastQCFastQC Report
Fri 10 Feb 2017
ERR1148137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856301
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117490.4113362002113923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58660.20537051242148502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58590.20512544021095816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82850.065.1112441
GGTATCA35300.059.2042271
TCAACGC110850.048.242074
CAACGCA113600.047.0742385
AACGCAG113600.047.0742386
ATCAACG113850.046.9708673
TATCAAC120550.044.7096182
ACGCAGA133200.040.192067
CGCAGAG136800.039.1343738
GCAGAGT149400.036.0329639
GTACATG150550.033.9729041
TACATGG154450.033.009042
ACATGGG153150.032.8202323
CATGGGG93650.030.4249364
ATGGGGG55700.030.3294895
AGAGTAC141800.030.16161510-11
CATGGGT35700.029.3255654
GAGTACT62200.025.53423512-13
AGTACTT68100.025.50572812-13
TGGGGGG48050.025.502096
Produced by FastQC (version 0.11.5)