FastQCFastQC Report
Fri 10 Feb 2017
ERR1148136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526878
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82640.3270438857752531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45990.18200324669414195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42120.16668790499580907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71950.057.7761881
GGTATCA36550.046.7647631
AACGCAG92100.044.6954576
TCAACGC93350.044.096974
CAACGCA94900.043.376735
ATCAACG97400.042.2633673
TATCAAC102400.040.609582
ACGCAGA107650.038.2944767
CGCAGAG110950.037.1018648
GTACATG124650.036.3116151
ACATGGG123400.035.3832633
TACATGG129050.034.8970872
GCAGAGT122200.033.8322229
CATGGGG72350.031.3258864
AGAGTAC115800.028.20205110-11
ATGGGGG36750.027.1937375
CATGGGT27900.027.0779914
CAGAGTA119450.024.4767810-11
AGTACTT52400.023.4993612-13
GAGTACT49700.023.33969512-13
Produced by FastQC (version 0.11.5)