FastQCFastQC Report
Fri 10 Feb 2017
ERR1148134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763411
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75410.4276371191968293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.23057585554360271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40190.2279105665100195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.069.574531
GTATCAA52450.059.4922641
TCAACGC66600.046.354014
ATCAACG68050.045.366313
CAACGCA68150.045.1251755
AACGCAG69450.044.194856
TATCAAC74100.041.9869842
ACGCAGA79800.038.5373547
CGCAGAG82950.036.930498
GCAGAGT90500.033.9152769
GTACATG78450.031.5014021
ACATGGG77800.031.2707233
TACATGG80650.030.6845742
ACCGTAG1055.9166865E-428.3253278
CATGGGG43300.028.0244124
AGAGTAC86250.027.86226810-11
GAGTACT44100.027.78579712-13
CAGAGTA89400.026.51459510-11
GTACTTT50050.024.30431714-15
AGTACTT48000.024.22700712-13
Produced by FastQC (version 0.11.5)