FastQCFastQC Report
Fri 10 Feb 2017
ERR1148132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846360
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.41199982668602003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39820.21566758378647718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.19611560042461923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51900.061.6129761
TCAACGC66650.047.1250574
AACGCAG67400.046.7771846
GGTATCA25850.046.5324671
ATCAACG68500.045.8523373
CAACGCA69300.045.4946945
TATCAAC77200.041.073652
ACGCAGA77100.040.892127
CGCAGAG79800.039.434018
GTATACG1156.6187204E-736.246451
GCAGAGT87300.036.0462049
GTACATG79400.035.8486371
TACATGG82850.034.107862
CATGGGT21050.033.9115224
ACATGGG82450.033.765513
AGAGTAC81600.030.2899810-11
CATGGGG46100.029.4206354
AGTACTT44550.026.90577112-13
CAGAGTA84650.025.72007610-11
CTTAGCG2151.235585E-724.901313
Produced by FastQC (version 0.11.5)