FastQCFastQC Report
Fri 10 Feb 2017
ERR1148131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263178
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37710.2985327483537554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.1605474446198398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.1541350466838403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32250.057.2270621
GGTATCA16000.055.0695081
TCAACGC39950.045.859054
ATCAACG40750.044.8127823
CAACGCA41050.044.7750855
AACGCAG41250.044.4137956
TATCAAC46400.039.615582
ACGCAGA47300.038.6072047
CGCAGAG49700.036.7428748
GTACATG53850.035.599151
ACATGGG52800.034.585623
TACATGG55150.033.9774322
GCAGAGT54550.033.9122629
CTTAGCG1152.769662E-531.0344873
CATGGGG33000.030.2821334
AGAGTAC50650.028.42019510-11
CATGGGT12800.026.4884194
ATGGGGG16600.025.7997515
CAGAGTA52550.025.7513410-11
GAGTACT24000.024.41271612-13
Produced by FastQC (version 0.11.5)