FastQCFastQC Report
Fri 10 Feb 2017
ERR1148130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478570
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.35716942721683786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.19376830315778082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27660.18707264451463237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36550.056.696791
GGTATCA18350.051.272871
TCAACGC44600.046.279754
CAACGCA45150.045.8477365
AACGCAG45700.045.165796
ATCAACG46650.044.3735283
ACGCAGA52400.039.50437
TATCAAC53850.038.3301162
CGCAGAG55050.037.710698
TATAGCG957.4879554E-637.5685165
GTACATG57200.035.187411
ACATGGG57450.033.5468183
GCAGAGT61900.033.5375379
TACATGG60500.032.4455342
CATGGGG33350.031.034864
AGAGTAC56700.029.11228810-11
ATGGGGG17700.027.2212525
CAGAGTA58800.025.59408210-11
GTACCGT1401.058115E-425.492926
GAGTACT29600.024.31588212-13
Produced by FastQC (version 0.11.5)