FastQCFastQC Report
Fri 10 Feb 2017
ERR1148129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556080
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69530.27201809020061973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.13923664361052862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34840.13630246314669336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57150.056.4898531
AACGCAG69100.046.3132256
TCAACGC69700.045.999894
CAACGCA70100.045.7374085
ATCAACG71000.045.157643
GGTATCA27750.044.861181
ACGCAGA79050.040.4837957
TATCAAC81350.039.707912
CGCAGAG80850.039.4353378
GCAGAGT94250.033.9548459
GTACATG90900.031.9774131
ACATGGG89200.031.1424523
TACATGG92750.030.7866252
CATGGGG46000.030.1299844
AGAGTAC87400.028.5850310-11
TAATACT21300.024.5755864
CAGAGTA92900.024.07536910-11
GAGTACT42900.023.50245312-13
TAGGTCG2552.4885594E-823.3270935
AGTACTT53800.022.7211512-13
Produced by FastQC (version 0.11.5)