FastQCFastQC Report
Fri 10 Feb 2017
ERR1148128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926673
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76880.3990298301787589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39270.20382285940582548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.19520697077293345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59100.065.504781
GGTATCA26950.058.7854771
AACGCAG77950.048.841876
TCAACGC78150.048.792994
CAACGCA79400.047.9499175
ATCAACG80250.047.442043
TATCAAC84300.045.5873762
ACGCAGA90650.041.9991577
CGCAGAG94200.040.4163868
GCAGAGT103500.036.9571959
GTACATG94250.033.3024671
TACATGG97200.032.2540322
ACATGGG97250.031.686083
CATGGGG58500.030.9133554
AGAGTAC96650.030.55946710-11
ATGGGGG31000.028.5925545
CCGTTCG1254.8910966E-528.5541769
GAGTACT45250.028.06775712-13
AGTACTT48150.026.93323912-13
CAGAGTA100800.026.7636410-11
Produced by FastQC (version 0.11.5)