FastQCFastQC Report
Fri 10 Feb 2017
ERR1148127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193711
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88320.40260544802847775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.23166223809790806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.21014618607464705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.057.911821
GTATCAA79200.056.981021
AACGCAG104050.042.7621466
TCAACGC105000.042.5452044
ATCAACG106850.041.919923
CAACGCA107200.041.6720775
TATCAAC110800.040.7503852
ACGCAGA120000.037.1279147
CGCAGAG124000.035.930248
GCAGAGT132350.033.663399
GTACATG119450.030.6033131
TACATGG122600.030.0351522
ACATGGG120900.029.5205843
AGAGTAC126900.028.05449910-11
CATGGGG69600.027.605354
CAGAGTA130750.025.90908210-11
GAGTACT60350.025.72546612-13
ATGGGGG33150.025.4803165
AGTACTT62750.024.69414312-13
CATGGGT23100.024.2056034
Produced by FastQC (version 0.11.5)