FastQCFastQC Report
Fri 10 Feb 2017
ERR1148126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808245
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107400.3824452638569641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55660.19820207994672828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55570.1978815950887476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83350.060.6673741
GGTATCA40450.055.657841
AACGCAG107850.046.277386
TCAACGC109600.045.5384674
CAACGCA111600.044.7756655
ATCAACG112550.044.503443
TATCAAC118250.042.4138342
ACGCAGA125150.039.7852027
CGCAGAG129050.038.7672468
GCAGAGT143800.034.9976129
GTACATG135200.034.713841
TACATGG140350.033.149382
ACATGGG139750.033.1172873
CATGGGG71650.030.2212314
AGAGTAC134700.029.16971410-11
CAGAGTA139700.026.23078310-11
CATGGGA57850.026.0162164
GAGTACT65450.025.31293712-13
AGTACTT72150.024.89989912-13
ATGGGGG32850.024.2658985
Produced by FastQC (version 0.11.5)