FastQCFastQC Report
Fri 10 Feb 2017
ERR1148125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832950
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98900.3491060555251593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51060.18023614959671017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50970.1799184595562929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73350.058.614051
GGTATCA35850.052.6544081
TCAACGC96900.043.5207374
ATCAACG99550.042.6012273
CAACGCA99150.042.4131475
AACGCAG99150.042.4131476
TATCAAC107150.039.5809752
ACGCAGA116850.035.937667
CGCAGAG119450.035.205228
GTACATG128750.034.3641971
ACATGGG129850.033.5307243
TACATGG131350.033.4206922
GCAGAGT133200.031.7050139
CATGGGG71200.029.6566564
AGAGTAC123650.028.26100210-11
ATGGGGG37100.025.8122465
CATGGGA57350.024.9952474
GAGTACT56650.024.8316212-13
CAGAGTA130050.023.50858910-11
AGTACTT66950.023.45455412-13
Produced by FastQC (version 0.11.5)