FastQCFastQC Report
Fri 10 Feb 2017
ERR1148124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036375
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133720.440393561401342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.23389732822856202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.21542134947099748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100450.060.709141
GGTATCA47200.050.0901831
AACGCAG130400.045.9833346
TCAACGC133550.045.0323644
ATCAACG136050.044.2923163
CAACGCA136500.044.1462975
TATCAAC142150.042.6908872
ACGCAGA149050.040.189727
CGCAGAG153600.038.9604768
GCAGAGT165100.036.2827269
GTACATG152400.036.106971
ACATGGG154200.035.066873
TACATGG156900.034.8856432
CATGGGG88450.032.2147834
AGAGTAC156950.030.41593710-11
ATGGGGG43650.028.2100335
CATGGGT29750.027.593684
AGTACTT88750.027.34697312-13
CAGAGTA163000.025.56454310-11
GAGTACT81850.025.50973312-13
Produced by FastQC (version 0.11.5)