FastQCFastQC Report
Fri 10 Feb 2017
ERR1148123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244582
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77840.23990763679265928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42470.13089513533638542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39290.12109418100698334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72750.061.065711
TCAACGC91150.047.6382184
AACGCAG91900.047.119996
CAACGCA93600.046.3277325
GGTATCA33100.046.0578351
ATCAACG94600.045.9008873
TATCAAC106300.041.411532
ACGCAGA105950.041.039857
CGCAGAG108950.040.018998
GCAGAGT123000.035.544439
GTACATG126850.034.3177221
ACATGGG125400.033.6308633
TACATGG131250.032.58762
AGAGTAC115250.029.54771410-11
CATGGGG73850.029.3989454
ATGGGGG39050.027.2659935
CAGAGTA121100.024.16630710-11
CATGGGT31200.023.068544
AGTACTT67350.023.00697112-13
CATGGGA44450.022.2139434
Produced by FastQC (version 0.11.5)