FastQCFastQC Report
Fri 10 Feb 2017
ERR1148122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618345
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153210.5851406136318934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82080.31348046189482287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77400.2956065759095918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107250.061.086781
GGTATCA50600.047.4358141
AACGCAG140000.046.1421286
TCAACGC144900.044.745974
CAACGCA146750.044.141355
ATCAACG147450.044.0124783
TATCAAC150900.043.208272
ACGCAGA157300.040.9539267
CGCAGAG161000.039.9758038
GCAGAGT173450.037.3464559
GTACATG152150.032.725491
ACATGGG150550.031.7271183
AGAGTAC166300.031.6195610-11
TACATGG155950.031.3949682
CATGGGG87000.029.604964
AGTACTT88650.028.04745112-13
CAGAGTA170700.026.10021810-11
GAGTACT83550.025.523412-13
CATGGGT29500.024.8015564
AGTACAT112750.024.63747212-13
Produced by FastQC (version 0.11.5)