FastQCFastQC Report
Fri 10 Feb 2017
ERR1148121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520153
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63600.41837893948832783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33140.21800437192835195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.21320222372353312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45450.059.7612951
GGTATCA22700.053.0047261
TCAACGC60850.044.287744
CAACGCA61600.043.6519475
ATCAACG62000.043.466273
AACGCAG61250.043.4157526
TATCAAC67900.040.1300932
ACGCAGA69500.038.2620857
CGCAGAG72100.036.7998058
GCAGAGT79200.033.4257169
GTACATG75050.032.0641481
TACATGG76150.031.5635592
ACATGGG75700.030.8846443
AGAGTAC74750.028.77020610-11
CATGGGT20900.028.17964
CATGGGG40800.026.9747524
GAGTACT34100.025.38367712-13
AGTACTT37250.025.15360312-13
CAGAGTA77100.025.0383810-11
GCCGGTC10200.023.3295158
Produced by FastQC (version 0.11.5)