FastQCFastQC Report
Fri 10 Feb 2017
ERR1148120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192315
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48780.40912007313503557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.23055987721365576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.2032181093083623No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14210.11917991470374859No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12830.10760579209353233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40400.057.1905941
GGTATCA20600.050.2986451
TCAACGC53950.042.8920564
ATCAACG55200.042.0285343
AACGCAG55000.041.965056
CAACGCA55250.041.6674925
TATCAAC58850.039.7284132
ACGCAGA63750.036.1118287
CGCAGAG65500.035.0561878
GTACATG71650.034.4079171
CATGGGT24350.033.957414
TACATGG72650.033.9015732
ACATGGG72150.033.4740643
GCAGAGT71550.032.175119
ATGGGTA10150.028.7176385
CATGGGG31850.028.2024124
AGAGTAC67100.027.48260310-11
ATGGGTG12350.024.5652895
CAGAGTA69150.024.13011610-11
GGGTACC6950.023.9658017
Produced by FastQC (version 0.11.5)