FastQCFastQC Report
Fri 10 Feb 2017
ERR1148119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264373
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.3131987158852649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23690.18736559543742234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.15849753197830072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30400.058.980021
GGTATCA15200.053.6894531
TCAACGC39300.044.9528434
CAACGCA39700.044.499925
AACGCAG39900.044.276866
ATCAACG40250.043.8918463
TATCAAC45500.038.9642942
ACGCAGA47850.036.7962077
CGCAGAG49850.035.439258
GCAGAGT55850.031.8450019
GTACATG49600.029.3035431
TACATGG52200.027.8087392
ACATGGG51850.027.1889763
AGAGTAC50850.026.495410-11
CAGAGTA53250.024.24004410-11
CATGGGG28800.023.9584544
GAGTACT26000.023.4500512-13
AGTACTT27400.023.3373312-13
TAGCACG1602.6165007E-422.3061454
ATGGGGG13000.021.5054135
Produced by FastQC (version 0.11.5)