FastQCFastQC Report
Fri 10 Feb 2017
ERR1148117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884477
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97220.3370455025295747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.17715516539046766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50830.1762191204852734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71950.061.329151
GGTATCA33900.048.4829371
AACGCAG92550.047.11626
TCAACGC92800.046.9892654
CAACGCA94200.046.227765
ATCAACG96150.045.2902263
TATCAAC106050.041.7937052
ACGCAGA110400.039.4982227
CGCAGAG113750.038.3872838
GCAGAGT126400.034.5455139
GTACATG116400.032.2305071
ACATGGG117850.030.9942743
TACATGG121800.030.576822
TATACCG2750.030.285735
AGAGTAC118450.029.50635110-11
CATGGGG69100.027.2051874
AGTACTT66500.024.60105312-13
CAGAGTA123050.024.55979710-11
GAGTACT59450.023.7659112-13
TATACTG14050.023.2878325
Produced by FastQC (version 0.11.5)