FastQCFastQC Report
Fri 10 Feb 2017
ERR1148116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519940
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95390.3785407589069581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.20369532607919233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48740.19341730358659331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78150.055.7758671
GGTATCA39300.053.6382941
AACGCAG97950.043.5407686
TCAACGC101350.041.9627234
CAACGCA102650.041.547185
ATCAACG103500.041.1485063
TATCAAC110350.039.1925742
ACGCAGA110000.038.7710767
CGCAGAG113300.037.4843258
GCAGAGT126450.033.868439
GTACATG115850.033.461791
TACATGG119800.032.0787472
ACATGGG118650.031.9841143
AGAGTAC117650.029.87974710-11
CATGGGG63600.027.9637534
CATGGGT27600.026.2925154
CAGAGTA123050.025.95816810-11
GAGTACT58850.025.36930812-13
CGTATAC1501.6909484E-423.792573
AGTACTT65950.023.72041912-13
Produced by FastQC (version 0.11.5)