FastQCFastQC Report
Fri 10 Feb 2017
ERR1148115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759860
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110900.2949577909815791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58040.1543674498518562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.14787784651556174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98250.057.634411
AACGCAG123750.045.472866
GGTATCA47550.045.4558751
TCAACGC126900.044.34414
CAACGCA129850.043.47415
ATCAACG130350.043.2617073
TATCAAC141500.040.1081772
ACGCAGA141000.039.740947
CGCAGAG144350.038.8598638
GCAGAGT163900.034.5150039
GTACATG158050.032.248771
ACATGGG157450.031.7353273
TACATGG162850.031.0507552
AGAGTAC153150.030.37358710-11
CATGGGG90300.029.8412344
ATGGGGG44600.026.5414455
AGTACTT80550.026.3638612-13
TTAGCGC3051.0913936E-1125.3541984
CATGGGT38050.025.3260064
CAGAGTA160050.024.34410-11
Produced by FastQC (version 0.11.5)