FastQCFastQC Report
Fri 10 Feb 2017
ERR1148114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406268
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106260.4415966966273084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.24656438933651614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.21714123281363507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77700.062.089911
GGTATCA36950.050.1306151
AACGCAG102250.046.1353876
TCAACGC105800.045.0371744
CAACGCA108200.043.983225
ATCAACG108600.043.766443
TATCAAC112950.042.4513132
ACGCAGA116600.040.5085037
CGCAGAG120050.039.3939258
GCAGAGT131550.036.221469
GTACATG124800.035.8411871
ACATGGG127550.034.4657943
TACATGG129550.034.394322
CATGGGT31200.032.0316284
CATGGGG72350.031.0797524
AGAGTAC124150.030.6420710-11
AGTACTT62850.027.35374812-13
TATACCG2205.173206E-927.0396845
ATGGGGG33900.026.1463365
CAGAGTA128800.025.63311610-11
Produced by FastQC (version 0.11.5)