FastQCFastQC Report
Fri 10 Feb 2017
ERR1148113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678574
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118050.44071957690920616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65010.24270376700438367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57550.21485312707433135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86750.058.7593461
GGTATCA41950.054.0836141
AACGCAG110200.045.7763146
TCAACGC113900.044.2370574
ATCAACG115750.043.4272423
CAACGCA116050.043.41755
TATCAAC124950.040.7564622
ACGCAGA127350.039.471567
CGCAGAG131200.038.2679448
GTACATG137900.035.280151
GCAGAGT145700.034.8678259
TACATGG141500.034.1816522
ACATGGG138600.034.0787623
CATGGGG73650.029.8851574
AGAGTAC137450.029.66806810-11
CATGGGT37450.028.7510224
AGTACTT70050.026.11340712-13
GAGTACT64450.025.79796412-13
CAGAGTA142400.025.67074410-11
ATGGGGG31500.025.3058415
Produced by FastQC (version 0.11.5)