FastQCFastQC Report
Fri 10 Feb 2017
ERR1148112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588675
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137330.5305030565830009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71980.27805730730972406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66260.2559610611606324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108600.054.9906271
GGTATCA59150.044.693651
AACGCAG140700.041.9387556
ATCAACG143100.041.4016533
TCAACGC143300.041.343874
CAACGCA145200.040.76195
TATCAAC151100.039.4882622
ACGCAGA160500.036.6538167
CGCAGAG162250.036.2584728
CATGGGT46600.034.3371544
ACATGGG162150.033.8229873
GCAGAGT174750.033.8010379
GTACATG171800.033.305641
TACATGG174500.032.727082
GTATACG1352.3194789E-630.8732151
CATGGGG81650.030.525014
AGAGTAC167850.028.31544710-11
ATGGGTG25800.028.127955
AGTACTT76500.026.74822612-13
ATGGGGG35200.026.0241185
Produced by FastQC (version 0.11.5)