FastQCFastQC Report
Fri 10 Feb 2017
ERR1148110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208573
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.1865836817469859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13570.1122811778849933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.10739938754216749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27350.066.165191
GGTATCA12350.056.875911
TCAACGC37400.048.039344
ATCAACG38200.047.189023
CAACGCA38900.046.1869245
AACGCAG39450.045.995426
TATCAAC41500.043.438442
ACGCAGA47250.038.1507037
CGCAGAG48600.036.9685488
GTACATG56350.034.226651
GCAGAGT53300.033.7086529
TACATGG60000.031.9289252
ACATGGG58550.031.803813
CATGGGG36400.031.217184
TATGTAA6550.029.0662062
ATGGGGG18650.028.390495
AGAGTAC47700.028.3119110-11
TGGGGGG14850.026.441086
TATATGT5000.026.1766684
ATATGTA6700.025.7653771
Produced by FastQC (version 0.11.5)