FastQCFastQC Report
Fri 10 Feb 2017
ERR1148109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2696625
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108040.4006489593473323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58670.21756825661706763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56040.20781532471144487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82900.060.349551
GGTATCA40900.050.676341
AACGCAG106850.045.93096
TCAACGC107750.045.7680854
ATCAACG110250.044.7302673
CAACGCA110150.044.7168665
TATCAAC116450.042.7087142
ACGCAGA124900.039.3407977
CGCAGAG129500.037.8974278
GTACATG133400.035.940931
TACATGG137500.034.9156842
GCAGAGT141600.034.8690729
ACATGGG135300.034.8219383
CATGGGG83300.031.3505424
AGAGTAC132700.029.1833810-11
ATGGGGG42600.027.5094645
CATGGGT28250.025.6901684
CAGAGTA137600.025.44213510-11
AGTACTT70600.024.81453512-13
GAGTACT66900.024.1862412-13
Produced by FastQC (version 0.11.5)