FastQCFastQC Report
Fri 10 Feb 2017
ERR1148108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217660
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89940.4055626200589811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46660.21040195521405447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45660.20589269770839533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66550.064.523731
GGTATCA30600.057.9998781
TCAACGC91700.046.4458434
ATCAACG92000.046.2297333
CAACGCA93950.045.2702035
AACGCAG95000.044.707236
TATCAAC97850.043.650212
ACGCAGA108050.039.3076027
CGCAGAG110250.038.5232358
GTACATG124450.037.5191841
TACATGG127600.036.456822
ACATGGG125150.036.360833
GCAGAGT119100.035.7106259
CATGGGG68350.033.1580964
ATGGGGG37450.032.7203985
CATGGGT24850.032.3154754
AGAGTAC113300.029.74220310-11
ATGGGAG20750.029.52725
TACCGTG2701.8189894E-1228.640647
AGTACTT54800.025.94301812-13
Produced by FastQC (version 0.11.5)