FastQCFastQC Report
Fri 10 Feb 2017
ERR1148107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692458
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119700.4445751800028078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61690.2291214941885816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61000.22655878011838995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96500.059.9874531
GGTATCA47950.052.910581
AACGCAG125050.045.763656
TCAACGC127900.044.883434
ATCAACG129500.044.237013
CAACGCA129900.044.0549965
TATCAAC135650.042.409992
ACGCAGA143150.039.8941427
CGCAGAG147800.038.598778
GCAGAGT159000.035.879869
GTACATG148500.033.6077231
TACATGG152000.032.5251242
ACATGGG151950.031.7895663
CATGGGG86800.029.881064
AGAGTAC151500.029.7243610-11
CATGGGT34950.028.0844574
ATGGGGG42100.026.9886115
GAGTACT69150.026.92659412-13
AGTACTT73800.026.92274712-13
CAGAGTA155850.026.50908510-11
Produced by FastQC (version 0.11.5)