FastQCFastQC Report
Fri 10 Feb 2017
ERR1148106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856796
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163520.5723894880838534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85460.2991463163628064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85070.2977811506316867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117100.059.2562141
GGTATCA58700.052.6616131
AACGCAG151400.045.0288856
TCAACGC159700.043.0238724
ATCAACG161100.042.502283
CAACGCA162650.042.2069745
TATCAAC166900.041.1707232
ACGCAGA170750.039.9260527
CGCAGAG172500.039.486528
GCAGAGT186800.036.686659
GTACATG168350.036.0870931
ACATGGG169600.034.900343
TACATGG175450.034.620652
CATGGGG97750.032.802314
CATGGGT38850.031.5434114
AGAGTAC179200.030.80650710-11
ATGGGGG44850.028.1193875
AGTACTT95150.027.7904412-13
CAGAGTA183100.027.01508710-11
GAGTACT89500.026.75317412-13
Produced by FastQC (version 0.11.5)