FastQCFastQC Report
Fri 10 Feb 2017
ERR1148105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570794
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160160.6229981865524815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83510.3248412747190168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.3152722466288625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110250.060.814891
GGTATCA52700.056.3819541
AACGCAG145900.045.5797426
TCAACGC150400.044.1368874
ATCAACG151250.043.96753
CAACGCA153700.043.266655
TATCAAC155150.043.2115362
ACGCAGA169750.039.2107967
CGCAGAG173050.038.4974338
GCAGAGT185750.035.9933979
GTACATG169000.035.9740371
TACATGG173400.035.0271032
ACATGGG170950.034.829753
CATGGGT43400.033.4414754
CATGGGG91700.031.8491234
GTATACG1004.4202415E-429.7728331
AGAGTAC178200.029.49063910-11
ATGGGGG40600.027.8363915
AGTACTT88750.027.44548412-13
GAGTACT86600.026.75314712-13
Produced by FastQC (version 0.11.5)