FastQCFastQC Report
Fri 10 Feb 2017
ERR1148104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744907
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70530.4042049232423275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37910.21726086261330832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.19829137025640908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63600.056.2613261
GGTATCA34650.044.8466341
AACGCAG85050.041.756026
TCAACGC86300.041.082284
ATCAACG86700.041.0299643
CAACGCA86800.040.7770965
TATCAAC91750.039.0332222
ACGCAGA96600.036.825037
CGCAGAG99050.035.7339948
GTACATG102850.032.8803631
GCAGAGT108450.032.801279
TACATGG104550.032.2628672
ACATGGG100750.032.2378923
CATGGGG53100.029.1271464
CATGGGT26800.028.8554344
AGAGTAC102150.027.95258110-11
AAGCGTA900.00954076726.4384845
AGTACTT46950.025.59380312-13
ATGGGGG22550.024.7970715
CAGAGTA103750.024.71201910-11
Produced by FastQC (version 0.11.5)