FastQCFastQC Report
Fri 10 Feb 2017
ERR1148103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122172
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56760.5058048142352509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.28783466349187115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26920.23989192387619723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35450.065.19851
GGTATCA16850.061.8671721
TCAACGC47750.048.087894
ATCAACG48650.047.3205683
CAACGCA49250.046.7440765
AACGCAG50100.046.0697446
TATCAAC51750.044.7237782
ACGCAGA57150.040.2825137
CGCAGAG58550.039.420918
GCAGAGT64000.036.0639729
GTACATG59150.032.528941
CATGGGT18350.031.4454254
TACATGG60450.031.2989732
ACATGGG59950.031.2567083
AGAGTAC60800.028.91183910-11
CAGAGTA62750.026.59138310-11
CATGGGG30500.026.3302974
GAGTACT28850.025.67115412-13
TAACGCA1200.001289488624.7862364
AGTACTT31000.024.75425312-13
Produced by FastQC (version 0.11.5)