FastQCFastQC Report
Fri 10 Feb 2017
ERR1148101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356302
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105230.4465896137252356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58170.2468698834020427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56780.24097080934447282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81900.060.1229671
GGTATCA41100.051.863281
AACGCAG107750.045.32326
TCAACGC109500.044.5988544
CAACGCA111050.043.922795
ATCAACG112000.043.6033443
TATCAAC115500.042.544952
ACGCAGA125750.038.740987
CGCAGAG128350.037.9561968
GCAGAGT140300.034.765699
GTACATG129400.034.281
TACATGG133100.032.673022
ACATGGG131600.032.4988253
CATGGGG72450.031.5273554
AGAGTAC132250.029.01073310-11
CATGGGT29250.026.4374
CAGAGTA135450.025.9539310-11
AGTACTT66500.025.85061312-13
GAGTACT62350.025.71088212-13
ATGGGGG32550.025.035965
Produced by FastQC (version 0.11.5)