FastQCFastQC Report
Fri 10 Feb 2017
ERR1148100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358508
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104770.44422151631455137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56320.23879503482710254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52130.221029566149447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82500.060.410081
GGTATCA41100.046.650031
AACGCAG107450.045.9451566
TCAACGC109700.045.1654624
CAACGCA110800.044.717075
ATCAACG111000.044.6900863
TATCAAC115550.043.241932
ACGCAGA123550.039.957977
CGCAGAG127750.038.6442878
GCAGAGT136950.036.1351059
GTACATG127700.033.991831
ACATGGG129950.032.68063
TACATGG131500.032.4783822
CATGGGG72150.031.409194
AGAGTAC130000.030.19733810-11
CATGGGT29450.028.47754
ATGGGGG33450.027.9171835
AGTACTT63900.027.50582712-13
GAGTACT59450.025.71280112-13
CAGAGTA134300.025.46595410-11
Produced by FastQC (version 0.11.5)