FastQCFastQC Report
Fri 10 Feb 2017
ERR1148099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972640
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77810.394446021575148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45090.22857693243572064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.2054607024089545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65600.057.1841771
GGTATCA34250.045.8968121
AACGCAG84650.043.7772756
TCAACGC88400.042.0547834
ATCAACG89650.041.4020583
CAACGCA91250.040.741295
TATCAAC92750.040.2167782
ACGCAGA96550.038.1351977
CGCAGAG99450.036.963358
GTACATG108400.035.2650871
ACATGGG107900.034.5096443
TACATGG111050.034.0715752
GCAGAGT109300.033.6866729
CATGGGG61750.033.136694
ATGGGGG32000.032.343495
AGAGTAC104450.029.38521610-11
CATGGGT22450.029.1449934
TGGGGGG27400.028.6556976
AGTACTT53000.026.26198812-13
CAGAGTA107200.024.55308710-11
Produced by FastQC (version 0.11.5)