FastQCFastQC Report
Fri 10 Feb 2017
ERR1148097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651561
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133960.5052118355942028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69480.2620343261950225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67510.25460474037746067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97300.062.801181
GGTATCA44800.051.713611
AACGCAG127250.047.3553126
TCAACGC128850.046.9057734
ATCAACG130250.046.4016083
CAACGCA132100.045.7067385
TATCAAC136000.044.7484862
ACGCAGA149300.040.361447
CGCAGAG155350.038.8278858
GCAGAGT166300.036.3785829
GTACATG156400.032.672611
TACATGG159200.032.0242772
ACATGGG155500.031.8280453
CATGGGG80350.029.2434254
AGAGTAC157550.029.129610-11
CATGGGT45050.027.9935594
AGTACTT76250.026.48603612-13
ATGGGGG36150.025.999555
CAGAGTA161850.025.83804510-11
TATACCG3101.2732926E-1124.9458735
Produced by FastQC (version 0.11.5)