FastQCFastQC Report
Fri 10 Feb 2017
ERR1148096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039319
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147130.72146633263359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.3803230392106385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75190.3687015126127889No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.10174965270269144No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20450.10027857338650796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104350.061.231831
GGTATCA50450.058.072961
AACGCAG138050.046.0642366
ATCAACG142050.044.7671053
TCAACGC142850.044.4747544
TATCAAC146100.043.8186652
CAACGCA145950.043.611625
ACGCAGA159850.039.7820937
CGCAGAG161850.039.2904978
CATGGGT45500.037.9148334
GCAGAGT172200.036.8944179
GTACATG163150.036.2801671
TACATGG165600.035.5690232
ACATGGG163400.035.1683
CATGGGG87800.032.656914
ATGGGGG42950.031.5787015
AGAGTAC167350.030.21452310-11
AGTACTT80850.028.62148312-13
ATGGGTA16250.027.4555685
CAGAGTA170800.027.1488110-11
Produced by FastQC (version 0.11.5)