FastQCFastQC Report
Fri 10 Feb 2017
ERR1148095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819725
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.3598767879471774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17930.21873189179297936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.19811522156820885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG208.4891735E-489.227639
GTATCAA19550.063.3657841
GGTATCA8750.062.6207161
TCAACGC25750.047.8190774
CAACGCA26400.046.6417165
ATCAACG26950.045.6898423
AACGCAG27700.044.66756
TATCAAC30600.040.244822
ACGCAGA32100.038.5448577
CGCAGAG33350.037.1001438
GCAGAGT36350.034.0382359
GTACATG32200.033.8480381
TACATGG33800.031.3302882
ACATGGG33300.030.3677623
AGAGTAC33400.029.47539310-11
CATGGGT9250.027.6525274
GTGTACG1306.334083E-527.488191
TATAGGG1755.7034595E-727.1964992
CATGGGG19300.027.1227344
CATTGCG1556.84151E-626.8642339
Produced by FastQC (version 0.11.5)