FastQCFastQC Report
Fri 10 Feb 2017
ERR1148094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583252
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68340.431643225462529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.2221377266537481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34410.21733748007266057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50150.062.107811
GGTATCA23400.058.027581
AACGCAG66250.046.778456
TCAACGC67350.046.1910744
ATCAACG69000.045.0865063
CAACGCA69150.044.9887055
TATCAAC73000.042.702892
ACGCAGA77450.040.167457
CGCAGAG80350.038.7917488
GCAGAGT86700.036.019219
GTACATG79900.033.2432561
TACATGG82600.031.6899452
AGAGTAC80700.030.95777310-11
ACATGGG83100.030.922653
CATGGGG47400.028.4866584
ATGGGGG21550.026.774325
CAGAGTA84100.026.48803910-11
AGTACTT40200.025.67245512-13
CATGGGT19100.025.537274
GAGTACT37750.025.13261612-13
Produced by FastQC (version 0.11.5)