FastQCFastQC Report
Fri 10 Feb 2017
ERR1148093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849755
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63790.34485648099342886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33450.18083475919783973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.1737527402277599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49500.064.962421
GGTATCA22200.054.4523541
TCAACGC66500.048.1243024
CAACGCA67200.047.534495
AACGCAG67550.047.4643146
ATCAACG67700.047.3591543
TATCAAC72550.044.278752
ACGCAGA80250.040.026957
CGCAGAG82200.039.1497738
GCAGAGT92250.035.078129
GTACATG88400.034.0856741
ACATGGG88750.033.043223
TACATGG92000.032.9131132
CATGGGG53500.029.5754684
CATGGGT20000.029.4448264
AGAGTAC85750.029.0312110-11
ATGGGGG29200.028.1125355
TACCGTC9900.025.2358477
AGTACTT39550.025.19254912-13
CAGAGTA88650.025.09554910-11
Produced by FastQC (version 0.11.5)